Prof. Zhongdong Qiao
Asso.Prof. Wangjie Xu
Project Description and Objectives
Previous studies have demonstrated testicular PEBP1 gene defects in male mice can lead to a significant decline (90%) in fertility. Our preliminary experiments also found that nicotine-induced hypermethylation of the Pebp1 gene leads to reduced expression levels, thereby affecting the ERK1 / 2 activity. PEBP1 expression levels have been positively correlated to abnormal sperm development, but little is known about the molecular mechanism. Based on the results, the project intends to research the PEBP1 regulation pathway, signaling pathways, molecule effectors, cellular events, in spermatogenesis events (such as migration, division, apoptosis, and sperm decapacitation using pebp1 over-expression), RNA interference, fluorescence in situ hybridization, spermatogonial stem cell transplantation, and knockout technical methods. Finally we will systematically explain PEBP1 regulation and mechanisms in spermatogenesis events on the cells through knockout mice levels in vivo.
The objective of the project is to investigate the effects of environmental factors and epigenetic mechanisms in spermatogenesis, provide molecular targets for clinical diagnosis and treatment of male infertility sperm. And its availability for innovative theory and practical applications.
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