Prof. Zhiping Xie
Project Description and Objectives
Antibodies are indispensable tools in modern biomedical research and applications. The yeast display method enables the selection and production of an antibody of interest in one single host system. It avoids the potential complications of different protein post-translational modifications when antibody isolation and production are performed in two hosts. The use of yeast also provides clear advantages in production cost and biosafety.
Clone, express and purify protein of interest.
Label protein of interest and isolate yeast cells displaying binding antibodies.
Purify antibodies and verify binding specificity.