Dr. Junsong Chen
Project Description and Objectives
Our lab focus on searching for drug targets which can inhibit tumor metastasis. The earlier work has indicated that AEP had great effect on breast cancer metastasis. Thus, the later work will focus on searching small molecule inhibitors of AEP, antitumor prodrug, and therapeutic antibody. In addition, the later work will provide in-depth on the mechanism of AEP in tumor microenvironment and tumor-associated macrophage.
The project 1# mainly focus on the mechanisms of tumor cell apoptosis or immunogenicity. Metastatic nodules will be digested to single cells by collagenase IV. We will purify the leukocytes through Percoll gradient from the Blood or tumor nodules. Flow cytometry: apoptosis, leukocyte subsets. The tumor nodules will be used for WB to study the mechanisms of tumor cell apoptosis or immunogenicity.
The project 2#: mainly focus on searching for the inhibitors of Bcl-2 (including natural products and derivatives, such as Ch282-5). Ch282-5 combined with hyperthermia suppress the hepatic metastasis of CT26 cells. Colon carcinoma CT26 cells were injected into the spleen of female Balb/c mice to establish hepatic metastasis. In the six weeks, we plan to study whether ch282-5 combined with hyperthermia could inhibit the growth of CT26 tumor in the liver.
The interested student should have a basic knowledge of Molecular biology technology.
Finish a research report.
Give two research presentations (a. references review; b. technical presentation).