Prof. Sheng-ce Tao
Project Description and Objectives
Tuberculosis is the No.1 most in infectious disease based on the direct and indirect mortality rates. Mycobacterium tuberculosis (Mtb) is the main causative agent of tuberculosis, which has caused 9.6 million newly identified active cases and 1.5 million deaths from the disease in 2014. Moreover, the emergence of multidrug resistant (MDR) and extensively drug resistant (XDR) strains of Mtb has increased the threat that this disease imposes on the public health throughout the globe.
For Mtb, ribosome is one of the most important targets for antibiotics development. Although the core structure of bacteria ribosome is similar to that of eukaryote, there are significant differences of the overall structure and regulation mechanisms. Consequently, the ribosome of bacteria has been used as a direct target for drug screening.
In lieu of the above situation, we will try to reveal the ribosome regulation systematically through global protein-protein interaction discovery. By using bioinformatics analysis and initial validation and function analysis, we aim to identify 1-2 Mtb specific ribosomal protein-protein interactions as potential drug targets.
This will be done by: 1) Constructing the first protein-protein interaction network for all the Mycobacterium tuberculosis (Mtb) ribosomal proteins. 2) Identifying 1-2 Mtb specific ribosomal protein-protein interaction as potential drug targets.
Basic knowledge of molecular biology and proteomics.
Skills recommended but not required: molecular cloning, protein purification.
Clone 2-3 ribosomal genes.
Analyse 2-3 ribosomal proteins.
Profile the global protein-protein interaction using the proteome microarray.
Construct a protein-protein interaction network for at least one ribosomal protein.