Dr. Wangjie Xu
Project Description and Objectives
Prior studies have demonstrated that testicular Pebp1 gene defects in male mice can lead to a significant decline (90%) in fertility. Our preliminary experiments have also found that nicotine-induced hypermethylation of pebp1 genes leads to reduced expression levels, thereby affecting the ERK1 / 2 activity. This is accredited to the fact that its expression level positively correlates with abnormal sperm development. However, little is known about the molecular mechanisms. Dependent on the results, the project intends to research the PEBP1regulational pathway, PEBP1- signaling pathways - molecule effectors - cellular events, in spermatogenesis events such as, migration, division, apoptosis and sperm decapacitation using pebp1 over-expression, RNA interference, fluorescence in situ hybridization, spermatogonial stem cell transplantation, and knockout technical methods. Finally, we will systematically explain why PEBP1 regulations and mechanisms in spermatogenesis events on the cells in vivo knockout mice levels. The goal of the project is to investigate the effects of environmental factors and epigenetic mechanisms in spermatogenesis, provide molecular targets for clinical diagnosis and treatment of male infertility sperm. It is available for innovative theory and practical application.
Interested students should have basic knowledge of molecular biology.
Finish a program and write a research report.
Give (technical) research presentations.